IEG Members:Joy Van Nostrand

EDUCATION:

2006    Ph.D.   Marine Biomedicine and Environmental Sciences Center; Medical University of South Carolina, Charleston, SC

Dissertation Advisor: Pamela J. Morris, Ph.D.; Dissertation: pH-Dependent Nickel Tolerance in Burkholderia vietnamiensis PR1₃₀₁

1999    M.S.    Clinical Laboratory Science, College of Health Professions; Medical University of South Carolina, Charleston, SC

Project Advisor: Alan Junkins, Ph.D.; Project: Poor Predictive Ability of Urinalysis and Microscopic Examination to Detect Infection

1998    B.S.    Medical Technology, College of Health Professions; Medical University of South Carolina, Charleston, SC

1995    A.A.S.    Medical Laboratory Technology, Kankakee Community College, Kankakee, IL

PROFESSIONAL EXPERIENCE:

2006-present    Postdoctoral Research Associate; Institute for Environmental Genomics, Dept. of Botany and Microbiology,The University of Oklahoma, Norman, OK

Responsibilities: Conduct research on microbial community functional changes at contaminated sites and with the use of different remediation strategies.

2000-2006    Graduate Research Assistant; Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, Charleston, SC.

Responsibilities: Conducted research on the effect of pH on nickel toxicity to Burkholderia vietnamiensis PR1₃₀₁ and on mechanisms of nickel tolerance.

2000    Laboratory Rotation Student; Microbiology and Immunology Department, Medical University of South Carolina, Charleston, SC, Laboratory of Michael Schmidt, Ph.D.

Responsibilities: Isolated plasmids from benzene, toluene, ethylbenzene, and xylene degrading isolates using alkaline lysis.

1999    Laboratory Rotation Student; Microbiology and Immunology Department, Medical University of South Carolina, Charleston, SC, Laboratory of Harold May, Ph.D.

Responsibilities: Monitored the effect of temperature on anaerobic degradation of polychlorinated biphenyls by microbial communities from Norway using gas chromatography coupled to electron capture detection.

1997-2000    Medical Laboratory Technician/Medical Technologist; Microbiology Department, LabCorp, Charleston, SC.

Responsibilities: Identified bacterial pathogens using manual (morphological, biochemical, serological tests) and automated methods. Performed and interpreted manual and automated antimicrobial sensitivities.

1996, Sept-Dec    Work Study Student; Coastal Carolina University, Conway, SC.

Responsibilities: Assisted Physics professor with lab set-up, lab instruction, and grading of student assignments. Prepared chemicals for Organic Chemistry Lab. Assisted library patrons in locating needed information.

1995-1996    Reuse Technician; BMA Conway Dialysis Center, Conway, SC.

Responsibilities: Prepared artificial kidneys for reuse following Universal Precautions. Maintained quality control records and assisted technical supervisor with inventory control.

ADDITIONAL LABORATORY EXPERIENCE:

2005, 6-20 July    Microbial Genomics and Ecology Group, Laboratory of Dr. Jizhong Zhou, Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN

Objectives: Utilized a functional gene microarray to detect metal related and degradative genes in Burkholderia vietnamiensis PR1301 and four Gram-positive microorganisms isolated from a metals contaminated riparian site.

2003, 18-21 Aug    Advanced Photon Source, Argonne National Laboratory, Argonne, IL

Objectives: Used the 2-ID-E beam-line to obtain elemental data on Burkholderia vietnamiensis PR1₃₀₁ under different growth conditions, determine if U could be detected using the 2-ID-E beam-line, and gather preliminary information on sorption of Ni and U to PR1.

AWARDS AND FUNDING:

2008    OCAST (OARS Program) Research grant, Co-PI with Liyou Wu, et al., Whole genomic DNA array for bacterial detection and identification, 3 years

2007    Student/Postdoctoral Travel Grant for the 15th Annual International Conference on Microbial Genomics, College Park, MD

2006-2007    Oak Ridge National Laboratory (ORNL) Postdoctoral Research Associates Program, provided salary and benefits

2006    Nominated, Distinguished Graduate Student Award, Medical University of South Carolina

2004-2005    Savannah River Ecology Laboratory (SREL) Graduate Research Participation Program, provided stipend support

2003, 12-16 Jan    Invited participant, U. S. EPA ORD Leadership Summit, Baltimore, MD

2002    The Corporate Partner Student Travel Grant, American Society for Microbiology

2001-2004    U.S. EPA STAR Fellowship for Graduate Environmental Study, provided tuition, stipend support and supply money

1999-2001    Graduate Student Fellowship, College of Graduate Studies, MUSC, Charleston, SC.

1998, 1999    Dean’s Honor Roll, College of Health Professions, MUSC, Charleston, SC.

MEMBERSHIPS AND CERTIFICATIONS:

American Association for the Advancement of Science (AAAS), since 2008

American Society for Microbiology (ASM), since 2001

ASCP certified Medical Technologist #207572, since 1995

PUBLICATIONS:

Kimes, N. E., J. D. Van Nostrand, E. Weil, J. Zhou, P. J. Morris. Microbial functional structure of Montastraea faveolata, an important Caribbean reef-building coral, differs between healthy and Caribbean yellow-band diseased colonies. Envrion. Microbiol. In press.

Liang, Y., G. Li, J. D. Van Nostrand, Z. He, L. Wu, Y. Deng, X. Zhang, J.Zhou. 2009. Microarray-based analysis of microbial functional diversity along an oil contamination gradient in oil field. FEMS Microbiol. Ecol. doi: 10.1111/j.1574-6941.2009.00774.x

Van Nostrand, J. D., W.-M. Wu, L. Wu, Y. Deng, J. Carley, S. Carroll, Z. He, B. Gu, J. Luo, C. Criddle, D. B. Watson, P. M. Jardine, T. L. Marsh, J. M. Tiedje, T. C. Hazen, J. Zhou. 2009. GeoChip-based analysis of functional microbial communities during the reoxidation of a bioreduced uranium-contaminated aquifer. Environ. Microbiol. doi:10.1111/j.1462-2920.2009.01986.x

Waldron, P. J., L. Wu, J. D. Van Nostrand, C. Schadt, D. Watson, P. Jardine, T. Palumbo, T. C. Hazen, J. Zhou. 2009. Functional gene array-based analysis of microbial community structure in groundwaters with a gradient of contaminant levels. Environ. Sci. Technol.43:3529–3534.

Wang, F., H. Zhou, J. Meng, X. Peng, L. Jiang, P. Sun, C. Zhang, J. D. Van Nostrand, Y. Deng, Z. He, L. Wu, J. Zhou , X. Xiao. 2009. GeoChip-based analysis of metabolic diversity of microbial communities at the Juan de Fuca Ridge hydrothermal vent. PNAS. 106:4840-4845.

Liang, Y., J. Wang, J. D. Van Nostrand, J. Zhou, X. Zhang, G. Li. 2009. Microarray-based functional gene analysis of soil microbial communities in ozonation and biodegradation of crude oil. Chemosphere. 75:193–199.

Mason, O. U., Di C. A DiMeo-Savoie, J. D. Van Nostrand, J. Zhou, M. R. Fisk, and S. J. Giovannoni. 2009. Prokaryotic diversity, distribution, and preliminary insights into their role in biogeochemical cycling in marine basalts. ISME J. 3:231-42.

Van Nostrand, J. D., J. Arthur, L. Kilpatrick, B. Neely, P. M. Bertsch, and P. J. Morris. 2008. Changes in protein expression in Burkholderia vietnamiensis PR1₃₀₁ at pH 5 and 7 with and without nickel. Microbiology. 154:3813-3824.

Deng, Y., Z. He, J. D. Van Nostrand, J. Zhou. 2008. Design and analysis of mismatch probes for long oligonucleotide microarrays. BMC Genomics. 9:491-503.

Wu, W. M., J. Carley, J. Luo, M. A. Ginder-Vogel, E. Cardenas, M. B. Leigh, C. Hwang, S. D. Kelly, C. Ruan, L. Wu, J. Van Nostrand, T. Gentry, K. Lowe, T. Mehlhorn, S. Carroll, W. Luo, M. Fields, B. Gu, D. Watson, K. M. Kemner, T. Marsh, J. Tiedje, J. Zhou, S. Fendorf, P. K. Kitanidis, P. M. Jardine, and C. S. Criddle. 2007. In situ bioreduction of uranium (VI) to submicromolar levels and reoxidation by dissolved oxygen. Environ. Sci. Technol. 41:5716-5723.

Van Nostrand, J. D., T. V. Khijniak, T. J. Gentry, M. T. Novak, A. G. Sowder, J. Z. Zhou, P. M. Bertsch, and P. J. Morris. 2007. Isolation and characterization of four Gram-positive nickel-tolerant microorganisms from contaminated sediments. Microb. Ecol. 53(4):670-682.

Van Nostrand, J. D., T. J. Khijniak, B. Neely, M.A. Sattar, A. G. Sowder, G. Mills, P. M. Bertsch, and P. J. Morris. 2007. The use of hydroxylapatite to reduce the toxicity of nickel and uranium to Burkholderia vietnamiensis PR1₃₀₁. Environ. Sci. Technol. 41(6):1877-1882.

Van Nostrand, J. D., A. G. Sowder, P. M. Bertsch, and P. J. Morris. 2005. The effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁. Environ. Toxicol. Chem. 24(11):2742-2750.

Van Nostrand, J. D., A. D. Junkins and R. K. Bartholdi. 2000. Poor predictive ability of urinalysis and microscopic examination to detect infection. Am. J. Clin. Pathol. 113:709-713.

INVITED REVIEWS AND CHAPTERS:

Van Nostrand, J. D., Z. He, and J. Zhou. Microarray analysis of environmental samples. In: Environmental Microbiology: Current Technology and Water Applications. Horizon Scientific, Norwich, UK, pp to be determined. (Invited book chapter, Submitted).

Van Nostrand, J. D., Z. He, and J. Zhou. Analysis of microbial communities by functional gene Arrays. In: L. Barton, M. Mandl and A. Loy (eds) Geomicrobiology: Molecular and Environmental Perspective. Springer, New York, (Invited book chapter, In press).

Van Nostrand, J. D, L. Wu, Z. He, and J. Zhou. GeoChips for analysis of microbial functional communities. In: J. R. van der Meer (ed) Handbook of Hydrocarbon and Lipid Microbiology, vol 4, Experimental Protocols and Appendices. Springer, New York, pp to be determined. (Invited book chapter, In press)

Van Nostrand, J. D, Y. Liang, Z. He, G. Li,and J. Zhou. GeoChip: A high throughput genomic tool for linking community structure to functions . In: K. N. Timmis (ed) Handbook of Hydrocarbon and Lipid Microbiology, vol 3, Consequences of microbial interactions with hydrocarbons, oils, and lipids. Springer, New York, pp to be determined. (Invited book chapter, In press)

He, Z., J. D. Van Nostrand, L. Wu, J. Zhou. 2008. Development and application of functional gene arrays for microbial community analysis. Transactions of Nonferrous Metals Society of China. 18: 1319-1327 (Invited review)

MANUSCRIPTS IN REVIEW:

Parnell, J., G. Rompato, L. Latta, M. Pfrender, J. D. Van Nostrand, Z. He, J. Zhou, G. Andersen, P. Champine, B. Ganesan, B. Weimer, Functional Biogeography as Evidence of Gene Transfer in Hypersaline Microbial Communities. (Submitted, Proc. Nat. Acad. Sci. USA)

Van Nostrand*, J. D., L. Wu*, W.M. Wu, T. J. Gentry, Z. Huang, Y. Deng, J. Carley, S. Carroll, Z. He, B. Gu, J. Luo, C. S. Criddle, D. B. Watson, P. M. Jardine, T. L. Marsh, J. M. Tiedje, T. C. Hazen, J. Zhou. Dynamics of microbial community composition and function correlate with changes in site geochemistry during the in situ bioremediation of a uranium-contaminated aquifer. (Submitted, ISME J.)*Authors contributed equally to this work

Zhili, H., M. Xu, Y. Deng, S. Kang, L. Kellogg, L. Wu, J. D. Van Nostrand, S. E. Hobbie, P. B. Reich, and J. Zhou. Metagenomic analysis reveals a marked divergence in the functional structure of belowground microbial communities at elevated CO₂. (Submitted, Ecol. Lett.)

Mason, O. U., T. Nakagawa, M. Rosner, J. D. Van Nostrand, J. Zhou, A. Maruyama, M. R. Fisk, S. J. Giovannoni, and the Integrated Ocean Drilling Program Expeditions 304/305 Science Party. Hydrocarbon utilizing prokaryotes in plutonic ocean crust sampled from the Atlantis Massif, 30 °N, 42 °W. (Submitted, Environ. Microbiol.)

PRESENTATIONS AND ABSTRACTS AT NATIONAL AND REGIONAL MEETINGS:

Van Nostrand, J. D., L. Wu, G. M. King, Z. He, J. Zhou. 2009. Microbial community succession after volcanic eruption. American Society for Microbiology General Meeting, 17 – 21 May, Philadelphia, PA (Poster presentation)

Van Nostrand, J.D., P.J. Waldron, D. B. Watson, Z. He, L. Wu, P. Jardine, T. C. Hazen, J. Zhou. 2009. GeoChip analysis of groundwater microbial communities across a gradient of pH, heavy metal, and nitrate contamination. American Society for Microbiology General Meeting, 17 – 21 May, Philadelphia, PA (Poster presentation)

Van Nostrand, J. D., P. J. Waldron, W-M. Wu, L. Wu, Y. Deng, J. Carley, Z. He, B. Gu, J. Luo, C. Criddle, P. Jardine, T. C. Hazen, J. Zhou. 2009. GeoChip-based analysis of functional microbial communities in a bioreduced uranium-contaminated aquifer during nitrate exposure. American Society for Microbiology General Meeting, 17 – 21 May, Philadelphia, PA (Poster presentation).

Van Nostrand, J. D., L. Wu, Zhili He, and J. Zhou. 2008. Use of Bst DNA polymerase for whole genome amplification. American Society for Microbiology General Meeting, 1-5 June, Boston, MA. (Poster presentation)

Van Nostrand, J. D., W-M. Wu, L. Wu, J. Y. Deng, Carley, S. Carrol, Z. He, B. Gu, J. Luo, C. Criddle, P. Jardine, T. C. Hazen, and J. Zhou. 2008. Microbial functional community changes in a bioreduced, uranium-contaminated subsurface during periods of resting, reoxidation, and recovery. American Society for Microbiology General Meeting, 1-5 June, Boston, MA. (Poster presentation)

Van Nostrand, J. D., L. Wu, S. Kang, P. Waldron, Y. Liang, Y. Deng, Z. He, W. Wu, H. Gough, S. Carroll, C. Schadt, A. Palumbo, D. Watson, C. Criddle, P. Jardine, B. Baldwin, A. Peacock, P. Long, D. Stahl, T.C. Hazen, J. Zhou. 2007. Applications of GeoChip to examine functional microbial communities in metal contaminated environments. Genomics: GTL Awardee Workshop VI, 10-13 Feb, North Bethesda, MD. (Poster presentation)

Van Nostrand, J. D., L. Wu, S. Carroll, Z. He, C. Criddle, P. Jardine, T. C. Hazen, and J. Z. Zhou. 2007. Changes in microbial community Function During a Period of Reoxidation in a Groundwater Recirculation System. 15th Annual International Conference on Microbial Genomics, 16-20 Sept, College Park, MD. (Poster presentation)

Van Nostrand, J. D., Y. Liang, L. Wu, S. Carroll, Z. He, C. Criddle, P. Jardine, T. C. Hazen, and J. Z. Zhou. 2007. Changes in Microbial Community Function During a Period of Reoxidation in a Groundwater Recirculation System. American Society for Microbiology General Meeting, 20-24 May, Toronto, Canada. (Poster presentation)

Neely, B. A., J. D. Van Nostrand, J. M. Unrine, P. M. Bertsch, and P. J. Morris. 2006. Investigating the effect of manufactured ZnO nanoparticles on Burkholderia vietnamiensis PR1301. American Society for Microbiology General Meeting, 21-25 May, Orlando, FL. (Poster presentation)

Van Nostrand, J. D., A. G. Sowder, P. M. Bertsch, and P. J. Morris. 2005. Ni tolerance in Burkholderia cepacia PR1₃₀₁. SREL Student Symposium, 15 July, Savannah River Ecology Laboratory, Aiken, SC. (Poster presentation)

Van Nostrand, J. D., A. Sowder, J. Arthur, P. M. Bertsch, and P. J. Morris. 2005. Ni tolerance in Burkholderia cepacia PR1₃₀₁, American Society for Microbiology General Meeting, 5-9 June., Atlanta, GA. (Poster presentation)

Van Nostrand, J. D., J. Arthur, P. M. Bertsch, and P. J. Morris. 2004. Protein expression changes in Burkholderia cepacia PR1₃₀₁ with exposure to nickel at pH 5 and 7. EPA STAR Graduate Fellowship Conference, 12-13 Oct, Washington, DC. (Poster presentation)

Van Nostrand, J. D., M. Swartz, J. Arthur, A. Sowder, P. M. Bertsch, and P. J. Morris. 2003. The effect of nickel and pH on protein expression in Burkholderia cepacia PR1₃₀₁. American Society for Microbiology General Meeting, 18-22 May, Washington, DC. (Poster presentation)

Van Nostrand, J. D., T. Khijniak, A. Sowder, P. Bertsch, P. J. Morris. 2003. The use of hydroxylapatite to reduce the toxicity of nickel and uranium to Burkholderia cepacia PR1₃₀₁. Carolinas Society of Environmental Toxicology and Chemistry Regional Meeting, 2-4 Apr, Charleston, SC. (Oral presentation)

Sowder, A. G., T. V. Khijniak, J. Van Nostrand, P. M. Bertsch, and P. J. Morris. 2002. Gram-Positive nickel resistant bacteria isolated from riparian sediments contaminated with Ni and U on the Savannah River Site. American Geophysical Union Fall Meeting, 6-10 Dec, San Francisco, CA. (Poster presentation)

Van Nostrand, J. D., T. V. Khijniak, A. Sowder, P. M. Bertsch, and P. J. Morris. 2002. Use of hydroxyapatite to alleviate U and Ni toxicity to microorganisms. American Society for Microbiology General Meeting, 19-23 May, Salt Lake City, UT. (Poster presentation)

Khijniak, T., J. Van Nostrand, M. Novak, A. Sowder, P. Bertsch, and P. Morris. 2000. Impact of nickel on microorganisms in the Steed Pond Corridor at the Savannah River Site. U.S. DOE EPSCoR Workshop, Lawrence Berkeley National Laboratory, 17-18 Aug, Berkeley, CA. (Poster presentation)

Van Nostrand, J. D., T. V. Khijniak, A. Sowder, P. M. Bertsch, and P. J. Morris. 2001. The impact of pH on microbial Ni toxicity. American Society for Microbiology General Meeting, 21 May, Orlando, FL. (Poster presentation)